Study on restoration of bFGF gene for rabbit internal carotid artery aneurysm wall
- Author:
- No author available
- Journal Title:
- Chinese Journal of Neurosurgery
- Issue:
- 10
- DOI:
- 10.3760/cma.j.issn.1001-2346.2012.10.031
- Key Word:
- 碱性成纤维细胞生长因子;慢病毒属;动脉瘤;模型,动物;Basic fibroblast growth faltor;Lentivirus;Aneurysm;Models,animal
Abstract： Objective To observe the effect of bFGF on the repair of aneurysm wall by injecting recombinant lentivirus with bFGF to the aneurysm model.Methods Injected lentivirus drops carrying bFGF daily from the rabbit ear after models were found.Tbe experiment was divided into two parts.Rabbits were killed each two week to collect samples.The expression of smooth muscle SM22α mRNA and HRG - 1 mRNA was detected by Real - time PCR.The PDGF expression of aneurysm wall was observed by immunohistochemical label.Results The aneurysms intervented by lentiviral drops had smaller size than the control group,with lighter degree of elastic fiber fracture and a larger number of smooth muscle cells.The expression of smooth muscle SM22c mRNA and HRG - 1mRNA was reduced.The PDGF of aneurysm wall PDGF expression showed an increasing trend.Conclusions Recombinant lentivirus carrying bFGF can repair damaged aneurysms and inhibit the aneurysm dynamic growth.It provides an experimental basis for conservative treatment of intracranial aneurysms in the future.
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