You Position: Home > Paper
Study on the differentially expressed genes of traumatic human cerebral cortex by genechip
( views:220, downloads:64 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROSURGERY
- Issue:
- 7
- DOI:
- 10.3760/j.issn:1001-2346.2007.07.004
- Key Word:
- 基因芯片;差异表达;颅脑创伤
Abstract： 目的 检测人创伤大脑皮层与正常大脑皮层基因表达谱的差异,筛选差异表达基因,寻找脑创伤基因治疗的新靶点.方法 利用含21 329个基因的人全基因组寡核苷酸芯片,检测5例创伤大脑皮层组织与1例正常对照的差异表达基因.并随机挑选2个差异表达基因做定量聚合酶链反应(PCR)验证.结果 5例创伤脑组织共同差异表达的基因数为87个,其中上调的60个,有16个功能未知或为表达序列标签(ESTs)片段,44个分属于12类功能;下调基因27个,有20个功能未知或为EST片段,7个分属于4类功能.定量PCR验证结果提示芯片结果可信.结论 基因芯片筛选出的人创伤大脑皮层的差异表达基因大部分在以前的颅脑创伤文献中未曾报道,对其干预有望成为颅脑创伤基因治疗的新靶向.
- [1]Li Z,Zhao X,Zhou Y,etc.Genomic analysis of anti-hepatitis B virus (HBV) activity by small interfering RNA and lamivudine in stable HBV-producing cells.[J].2005,79(22).
- [2]Ahmed F,Natale JE,Stoica B,etc.Gene expression profile changes are commonly modulated across models and species after traumatic brain injury.[J].2003,20(10).
- [3]Dash PK,Rall JM,Matzilevich DA.Comparative analysis of mRNA levels in the frontal cortex and the hippocampus in the basal state and in response to experimental brain injury.[J].2003,29(2).
- [4]Kluz T,Zhang Q,Davidson T,etc.Nickel-induced 1,4-alpha-glucan branching enzyme 1 up-regulation via the hypoxic signaling pathway.[J].2004,196(3).
- [5]Novarino G,Breit SN,Curmi PM,etc.Involvement of the intracellular ion channel CLIC1 in microglia-mediated beta-amyloid-induced neurotoxicity.[J].2004,24(23).
- [6]Li HH,Lee SM,Cai Y,etc.Differential gene expression in hippocampus following experimental brain trauma reveals distinct features of moderate and severe injuries.[J].2004,21(9).
- [7]Botez G,Sims J,Winckler J,etc.Spatial resolution of phospholipid scramblase 1 (PLSCR1), caspase-3 activation and DNA-fragmentation in the human hippocampus after cerebral ischemia.[J].2003,43(1).
- [8]Lee JH,Tsujimoto Y,Lee JH,etc.Reactive astrocytes express bis, a bcl-2-binding protein, after transient forebrain ischemia.[J].2002,175(2).