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Expression analyses of multiple DNA repair genes in glioma by TaqMan low-density array
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROSURGERY
- Issue:
- 2
- DOI:
- 10.3760/j.issn:1001-2346.2007.02.004
- Key Word:
- 神经胶质瘤;DNA修复;实时定量PCR
Abstract： 目的 运用低密度表达谱芯片检测人脑原发胶质瘤组织中DNA损伤修复基因的表达情况,进一步分析其表达变化的意义.方法 使用TaqMan低密度表达芯片技术检测27个DNA损伤修复基因在40例不同级别原发胶质瘤组织和10例正常脑组织中的表达情况,并通过统计学分析其在不同级别胶质瘤和正常脑组织中的表达差异.结果 与正常脑组织相比较,有13个DNA损伤修复基因在Ⅱ、Ⅲ、Ⅳ级胶质瘤中均表达下调,包括ERCC1、ERCC2、ERCC3、ERCC4、MGMT、MLH1、MLH3、NTHL1、OGG1、RAD50、SMUG1、XRCC4、XRCC5(P＜0.05).MSH2、MSH6、NUDT1和XRCC3只在Ⅱ级和Ⅲ级胶质瘤中表达下调;MRE11A和MUS81只在Ⅲ级和Ⅳ级胶质瘤中表达下调.PMS2、RAD52和XRCC1只在Ⅲ级胶质瘤中表达下调,而UNG只在Ⅱ级中表达下调.结论 TaqMan低密度表达芯片为多个基因的同时定量表达提供了一种准确、快速、有效的多变量检测技术,可用于发现新的肿瘤相关基因.大量的DNA损伤修复基因的表达下调,与胶质瘤的发生密切相关.
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