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Effect of profound hypothermia on early transcriptional changes of hippocampus after global ischemia in rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROSURGERY
- Issue:
- 11
- DOI:
- 10.3760/j.issn:1001-2346.2006.11.005
- Key Word:
- 深低温;脑保护;基因表达芯片;基因组学;大鼠
Abstract： 目的 筛选出深低温对全脑缺血大鼠海马影响的即早差异表达基因.方法 建立大鼠体外循环模型,实验分成两组,常温缺血组(n=3):常温条件下停循环全脑缺血5min;低温缺血组(n=3):深低温条件下停循环全脑缺血5min.采用Affymetrix大鼠全基因组芯片检测两组动物海马基因表达的变化,获取差异表达基因.结果 筛选出差异表达基因共有75个,其中39个基因表达有统计学意义(P＜0.01),上调33个,下调42个.结论 深低温对全脑缺血大鼠海马即早基因表达有明显影响,这些差异表达基因可能与深低温脑保护作用相关.
- [1]田鑫,江基尧,邱永明,等.大鼠深低温停循环复苏模型的建立[J].2004,(5).
- [2]江基尧,徐蔚,杨朋范,等.选择性脑超深低温技术对猴颈动脉血流阻断时限的研究[J].2003,(4).
- [3]Jorge Mendez,Jose Irarrazaval,Manuel Campos,etc.Hypothermic Circulatory Arrest in Cerebral Aneurysm Surgery[J].2002,12(3).
- [4]N, Croughwell,L R, Smith,T, Quill,etc.The effect of temperature on cerebral metabolism and blood flow in adults during cardiopulmonary bypass.[J].1992,103.
- [5]Tang Y,Clark JF,Ran R,etc.Genomics of the periinfarction cortex after focal cerebral ischemia.[J].2003,23(7).
- [6]Nishizawa Y..Glutamate release and neuronal damage in ischemia [Review][J].2001,69(4).
- [7]Tü,lin Alkan,Ahmet Bekar,etc.Hypothermia in Neuronal Protection[J].2001,11(2).
- [8]Miyazawa, T.,Bonnekoh, P.,Hossmann, K.-A..Temperature effect on immunostaining of microtubule-associated protein 2 and synaptophysin after 30 minutes of forebrain ischemia in rat[J].1993,85(5).