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Neural stem cell conditioned culture medium promotes corticospinal axons regeneration in injured rat spinal cord
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROSURGERY
- Issue:
- 10
- DOI:
- 10.3760/j.issn:1001-2346.2006.10.005
- Key Word:
- 神经干细胞;脊髓;损伤;培养基;鼠;胚胎
Abstract： 目的 研究神经干细胞条件化培养基对脊髓损伤大鼠(SCI)皮质脊髓束(CST)再生的促进作用.方法 成年雌性Wistar大鼠30只随机分为两组,神经干细胞条件化培养基治疗组和培养基对照组各15只,所有大鼠于T11水平横切脊髓.治疗组15只大鼠从固定在大鼠皮下Ommaya囊内注入神经干细胞条件化培养基,每周1次,每次注入5μl,对照组从Ommaya囊内注入同等量未培养过干细胞的培养基.利用Basso-Beattie-Bresnahan(BBB)评分客观评价后肢运动功能的恢复,检测生物素葡聚糖胺(Biotin dextran amine,BDA)示踪标记CST的再生,检测损伤部位远端的突触素表达情况.结果 所有大鼠在脊髓损伤后出现下肢截瘫,神经干细胞条件化培养基治疗组的大鼠表现为后肢运动功能的逐渐恢复,BDA标记的再生的轴突穿过了损伤处到达了脊髓的远端;对照组仅表现为轻微的组织和功能变化.治疗组大鼠脊髓在脊髓损伤处远端的神经元和BDA标记的轴突位置有突触素表达,对照组没有突触素表达.结论 神经干细胞条件化培养基可以促进SCI大鼠CST的再生以及CST和神经元之间的解剖学重建;神经干细胞治疗SCI可能通过支持治疗而非替代治疗.
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