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The research of the differential human cortex proteome after primary mechanical traumatic brain injury
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROSURGERY
- Issue:
- 2
- DOI:
- 10.3760/j.issn:1001-2346.2006.02.003
- Key Word:
- 差异蛋白质组学;脑创伤;双向电泳;生物质谱
Abstract： 目的应用蛋白质组学技术,研究不同程度脑创伤后的人脑皮层蛋白质组表达变化的情况.方法将临床标本以GCS评分分为中度和重度损伤组,提取脑挫伤部位皮层的总蛋白.通过双向电泳,分离蛋白.应用胶内酶切、生物质谱,鉴定由图像分析软件所得出的具有表达差异的蛋白质点.结果通过比较,目前已发现12个蛋白质点,表达水平具有显著性差异变化.在已鉴定出的蛋白点中,属于10种蛋白质.依其功能可分为:细胞骨架、代谢反应、氧化应激反应、功能未知等几类.在重伤组中,一共有9个蛋白点、6种蛋白表达上调(α-烯醇化酶、磷酸丙糖异构酶、5'磷酸吡哆醇氧化酶、crystalin、stathmin-1、NP25);中度损伤组3个蛋白点、4种蛋白表达上调(谷氨酰胺S转移酶、5'3'核苷酸酶、HSPC108、proapolipoprotein).结论外伤后,神经系统由于受伤程度的不同,蛋白表达水平会发生变化.原发性脑创伤的损伤程度不同将产生神经系统病生理反应的差异.
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