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Effects of proliferation and apoptosis of C6 glioma cells with triplex forming oligonucleotides (TFO)
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROSURGERY
- Issue:
- 6
- DOI:
- 10.3760/j.issn:1001-2346.2005.06.002
- Key Word:
- 血小板源生长因子;增殖;凋亡;三链形成寡核苷酸;C6胶质瘤细胞
Abstract： 目的观察PDGF-B链基因三链形成寡核苷酸(triplex-forming oligonucleotide,TFO)对C6胶质瘤细胞增殖和凋亡的影响.方法应用流式细胞技术观察TFO对C6胶质瘤细胞PDGF-B、PCNA表达的影响,应用流式细胞技术观察TFO对C6胶质瘤细胞凋亡的影响.结果TFO对C6胶质瘤细胞PDGF-B、PCNA的表达有明显抑制作用,而且抑制作用存在浓度依赖性.TFO有明显诱导C6胶质瘤细胞细胞凋亡作用,而且诱导作用存在浓度依赖性.结论TFO抑制C6胶质瘤细胞细胞增殖,同时诱导C6胶质瘤细胞细胞凋亡.
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