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Effect of traumatic brain injury tissue on human embryonic neural stem cells
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROSURGERY
- Issue:
- 4
- DOI:
- 10.3760/j.issn:1001-2346.2005.04.005
- Key Word:
- 神经干细胞;脑创伤;增殖;分化
Abstract： 目的建立人胚胎来源的神经干细胞体外培养方法,初步研究挫伤脑组织提取液(TBITE)对人胚神经干细胞(HNSCs)存活、增殖能力和分化的影响.方法10周龄左右的胎儿大脑皮层细胞体外培养,神经干细胞增殖2～3代后,剔除生长因子,分别加入不同浓度的脑外伤病人的挫伤脑组织提取液,培养2-3周后,免疫细胞化学方法鉴定细胞分化后的类型.用Laemmli凝胶电泳法分析培养液上清.结果成功获得以神经球形式生长的人胚神经干细胞,Nestin表达阳性;与空白对照相比,TBITE组中的神经干细胞有明显的增殖,并分化为神经细胞、星形胶质细胞和少突胶质细胞.随着TBITE含量的增加,星形胶质细胞的比例增加.结论脑外伤的局部微环境可促进神经干细胞增殖和分化.
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