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The method and condition for the application of in vitro invasive model-Boyden chamber to the invasive pituitary adenomas
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROSURGERY
- Issue:
- 3
- DOI:
- 10.3760/j.issn:1001-2346.2005.03.006
- Key Word:
- 垂体腺瘤;侵袭性;Boyden小室
Abstract： 目的探讨Boyden小室体外侵袭模型适用于侵袭性垂体瘤研究的实验条件和方法.方法选取手术切除的新鲜垂体瘤标本5例,体外进行原代培养,肿瘤在影像学上均为一侧或双侧的颈内动脉被完全包绕或肿瘤破入蝶窦的侵袭性表现.将不同浓度(0.5×105、1.0×105、1.5×105、2.0×105/ml)的垂体瘤细胞250μl置入Boyden小室侵袭模型,分别培养(6、12、18、24)h,观察不同浓度细胞在不同时间穿透基质凝胶多孔滤膜的侵袭率.结果在第6h,透过基质凝胶多孔滤膜的细胞还很少,随时间的延长而增多,在12h前后增幅最明显,18h以后有逐渐饱和的趋势;同时,随细胞浓度的增高,透过细胞比率也增加,但细胞浓度大于1.0×105/ml时,瘤细胞的侵袭百分率变化不显著.结论Boyden小室侵袭模型适用于原代培养垂体瘤细胞的条件是,在1.0×105/ml细胞浓度下培养12h后,计算细胞的侵袭率.
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