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Apoptosis in pituitary adenomas of human
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROSURGERY
- Issue:
- 3
- DOI:
- 10.3760/j.issn:1001-2346.2005.03.005
- Key Word:
- 垂体腺瘤;流式细胞;凋亡P53;Bcl-2;Bax
Abstract： 目的研究人垂体腺瘤中的细胞凋亡和凋亡相关基因(P53、Bcl-2、Bax)表达蛋白的情况,分析其和临床特征的相关性.方法采用流式细胞仪检测21例人垂体腺瘤中的凋亡和增殖细胞比例,采用TUNEL法检测14例细胞凋亡,同时应用免疫组化技术检测17例细胞增殖相关抗原(PCNA),凋亡相关基因P53、Bcl-2和13例Bax表达的蛋白产物.结果TUNEL法在14例垂体腺瘤中12例检测到细胞凋亡(占85.71%),其凋亡细胞比例0.1%～8.0%,流式细胞仪检测21例中凋亡细胞比例为1.77%±2.12%,两者和垂体腺瘤临床特征无明显相关性.凋亡相关基因中Bax阳性率高(16/17,92.31%),表达强度高,P53有2例阳性,Bcl-2仅1例阳性.Bax表达和细胞增殖活性相关显著.结论人垂体腺瘤中存在细胞凋亡,垂体腺瘤中Bax高表达,Bcl-2/Bax比值＜1.0,这一细胞内微环境有利于细胞凋亡的存在.垂体腺瘤的凋亡活性和其临床特征(如增殖活性,侵袭性等)无明显相关性.
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