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Measure of glioma relative gene expression by reverse dot-blot
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROSURGERY
- Issue:
- 5
- DOI:
- 10.3760/j.issn:1001-2346.2004.05.006
- Key Word:
- 胶质瘤;反向多点杂交;基因;基因芯片
Abstract： 目的利用反向点杂交技术检测胶质瘤相关基因谱在胶质瘤组织中的表达情况,为这些基因在胶质瘤恶性进展中的作用提供依据并找出具有进一步研究价值的基因,同时对基因芯片结果进行验证.方法分别提取正常脑和胶质瘤标本中的总RNA,并反转录为cDNA,随机引物法标记探针,与点于带正电荷尼龙膜上的基因片断进行反向杂交.用复日SmartView图像分析软件进行处理,将各个基因的表达强度和β-actine进行比对,根据比值作相关基因的半定量分析.结果Ⅱ级胶质瘤中表达上调的基因33条,下调19条,10条差异不明显;Ⅲ级胶质瘤中37条基因上调,17条基因下调,8条差异不明显;Ⅳ级胶质瘤中26条上调,20条下调,16条差异不明显.经单因素方差分析,差异具有统计学意义(P＜0.05)的有10、11、19、24、28、29和56号基因.结论反向点杂交可以同时检测数十个基因的表达,并对表达量高低进行半定量分析.通过这种方法我们发现eps8、A20 mRNA在胶质瘤中的高表达.其在胶质瘤中的高表达具有进一步研究的价值.
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