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Experimental study of inhibiting process of gene expression of Pgp in multidrug resistant rat glioma cell line (C6/adr) by antisense oligodeoxynucleotides
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROSURGERY
- Issue:
- 5
- DOI:
- 10.3760/j.issn:1001-2346.2004.05.002
- Key Word:
- 胶质瘤;大鼠;寡核苷酸;多药耐药性
Abstract： 目的通过研究反义寡核苷酸对大鼠胶质瘤多药耐药细胞株C6/adr中Pgp相关基因表达的抑制作用,探讨寡核苷酸在细胞内的代谢过程.方法根据MDR-1基因序列合成反义寡核苷酸转染胶质瘤耐药细胞,应用RT-PCR、流式细胞仪等方法,分别在转染后6、12、24、48h对其进行MDR-1mRNA水平及Pgp含量的检测.结果流式细胞及RT-PCR结果显示转染后6h即可发现胶质瘤耐药细胞Pgp和MDR-1 mRNA减低,减低程度与转染后的检测时间相关,12～24h减低程度最为显著(P＜0.05),48h后恢复至转染前的水平.结论反义寡核苷酸抑制大鼠胶质瘤细胞的PgP表达和减低MDR-1 mRNA水平具有明显的细胞内的代谢过程,了解其作用的规律性有助于选择适宜的用药时机,更大限度地提高疗效.
- [1]金澎,张庆林,刘福生,等.MGMT反义RNA对人脑胶质瘤耐药性逆转的研究[J].2003,(2).
- [2]尹格平,陈诵芬,孙晓明.卵巢癌组织及外周血淋巴细胞多药耐药基因表达蛋白的定量研究[J].1998,(5).
- [3]刘福生,张庆林,赵丽,等.胶质瘤多药耐药细胞系C6/adr的建立及其生物学特性的研究[J].2001,(2).
- [4]J G, Izant,H, Weintraub.Inhibition of thymidine kinase gene expression by anti-sense RNA: a molecular approach to genetic analysis.[J].1984,36(4).
- [5]A, Quattrone,L, Papucci,M, Morganti,etc.Inhibition of MDR1 gene expression by antimessenger oligonucleotides lowers multiple drug resistance.[J].1994,6.
- [6]R L, Juliano,V, Ling.A surface glycoprotein modulating drug permeability in Chinese hamster ovary cell mutants.[J].1976,455(1).
- [7]N, Kartner,J R, Riordan,V, Ling.Cell surface P-glycoprotein associated with multidrug resistance in mammalian cell lines.[J].1983,221.
- [8]刘炳仁,杨纯正,栾凤君,等.用逆转录-多聚酶链反应检测白血病患者多药耐药基因的表达[J].1995,(2).