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The co-inhibitory effect of p53 gene and PTEN gene on the growth of glioma cell line U251
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROSURGERY
- Issue:
- 1
- DOI:
- 10.3760/j.issn:1001-2346.2004.01.006
- Key Word:
- p53基因;PTEN基因;U251细胞系;凋亡
Abstract： 目的探讨p53基因和PTEN基因在脑胶质瘤细胞系U251发生发展过程中的作用机制.方法用不同MOI的p53腺病毒表达载体pAdCMV-p53及空载体pAdCMV-lacZ分别感染表达野生型PTEN基因和突变型PTEN基因的细胞系,RT-PCR及Western blot方法检测转染效率;并通过MTT检测生长抑制率、流式细胞仪检测细胞周期及TUNEL检测分析细胞凋亡等指标观察p53基因及PTEN基因对U251细胞生长的影响.结果MOI为100时,p53基因可引起U251细胞GoG1期阻滞、诱导细胞凋亡,生长抑制;MOI为50时,U251-p53+PTEN生长抑制率明显高于U251-p53,并能出现细胞凋亡,而U251-p53仅出现少量细胞凋亡.结论p53基因可以通过细胞周期GoG1期阻滞及诱导细胞凋亡抑制胶质瘤细胞系U251的生长;PTEN基因可以促进p53基因对胶质瘤细胞系U251的生长抑制作用,并能增加U251细胞对p53基因诱导凋亡的敏感性.
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