You Position: Home > Paper
Clone the genes associated with induced differentiation of glioma with RAP-DD
( views:176, downloads:12 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROSURGERY
- Issue:
- 2
- DOI:
- 10.3760/j.issn:1001-2346.2003.02.004
- Key Word:
- MIBP1;胶质瘤;细胞分化;RNA随机引物差异显示;基因表达
Abstract： 目的克隆胶质瘤细胞诱导分化相关新基因.方法应用RNA随机引物差异显示、SSCP纯化、PCR产物快速克隆、反Northern杂交及生物信息学分析等方法,观察人脑胶质瘤细胞株SHG-44-9分化过程中的基因表达变化.结果克隆了诱导分化前后表达差异显著的15个基因,其中诱导后下调基因4个,上调基因11个.同源性分析表明,5个是已知基因,10个是未知基因.诱导后上调的DIG-1基因与c-myc内含子结合蛋白1(MIBP1)基因高度同源,此基因具有转录因子活性,表达的蛋白可抑制c-myc基因的表达.结论克隆了15个人脑胶质瘤细胞诱导分化相关基因,其中控制c-myc基因表达的MIBP1基因诱导分化后上调提示,它可能是调控胶质瘤细胞分化基因,值得进一步研究.
- [1]孙立军,黄强.苯丁酸钠抗肿瘤的广谱诱导分化作用[J].2001,(6).
- [2]孙立军,张全斌,黄强.三株分化程度不同的胶质瘤细胞的建立[J].2002,(3).
- [3]李伟生,黄强,董军,等.苯丁酸钠诱导分化治疗可移植性人脑胶质瘤实验研究[J].2002,(2).
- [4]孙立军,黄强,王爱东,等.建立胶质瘤细胞诱导分化相关基因谱[J].2002,(3).
- [5]吴建强,李晓楠,黄强,等.环己亚甲基二乙酰胺对人胶质瘤细胞C-MYC和N-RAS表达的调控效应[J].1997,(4).
- [6]R, Makino,K, Akiyama,J, Yasuda,etc.Cloning and characterization of a c-myc intron binding protein (MIBP1).[J].1994,22.
- [7]Alexander Itkes,Carmen J Allegra,Maria Zajac-Kaye.Multiprotein complexes present at the MIF motifs flanking the promoter of the human c-myc gene[J].2000,474(1).
- [8]Xiao-Nan Li,Zi-Wei Du,Qiang Huang,etc.Growth-inhibitory and Differentiation-inducing Activity of Dimethylformamide in Cultured Human Malignant Glioma Cells[J].1997,40(6).
- [9]ZAJAC-KAYE Maria,BEN-BARUCH Noa,KASTANOS Evdokia,etc.Induction of Myc-intron-binding polypeptides MIBP1 and RFX1 during retinoic acid-mediated differentiation of haemopoietic cells[J].2000.
- [10]X N, Li,Z W, Du,Q, Huang.Modulation effects of hexamethylene bisacetamide on growth and differentiation of cultured human malignant glioma cells.[J].1996,84.
- [11]P, Liang,A B, Pardee.Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction.[J].1992,257(5072).