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The long-term culture and passaging of human neural stem cells
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROSURGERY
- Issue:
- 5
- DOI:
- 10.3760/j.issn:1001-2346.2002.05.005
- Key Word:
- 神经干细胞;人类;培养
Abstract： 目的探讨人类神经干细胞的体外培养条件及其传代的方法.方法采用机械方法从胎脑中分离神经细胞,应用N2培养基进行培养,bFGF和EGF刺激细胞扩增;传统方法和对神经球切割的方法进行传代培养;应用免疫组织化学染色对培养的细胞及其分化的细胞进行鉴定.结果从胎脑当中成功培养出人类的神经干细胞,培养条件下呈悬浮状态生长,形成神经球,绝大多数的细胞表达波形蛋白和Musashil两种神经干细胞的标志物;这种细胞可分化为神经元和星型胶质细胞,早期的培养有少量的少突胶质细胞;在这种培养条件下,神经干细胞生长速度较慢,而采用切割神经球的方法保持了细胞间的联系,神经干细胞可获得较大的扩增速度.结论在体外的培养条件下,可从胎脑组织中培养出神经干细胞,它可做为中枢神经系统疾病移植治疗的潜在细胞来源.
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