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Neural stem cells transplantation after traumatic brain injury improving functional outcome in adult rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROSURGERY
- Issue:
- 5
- DOI:
- 10.3760/j.issn:1001-2346.2002.05.004
- Key Word:
- 神经干细胞;颅脑外伤;神经移植
Abstract： 目的神经干细胞具有自我更新及多向分化潜能的特性,本文将人类神经干细胞移植到大鼠颅脑损伤的模型中,观察是否有治疗效果.方法人类神经干细胞取自3～4月胎儿海马,体重为300～370 g的大鼠制成脑损伤模型.伤后24小时将神经干细胞用立体定向注射法注入双侧顶叶皮层,伤后1周动物运动神经功能评分后,处死取脑,行病理及免疫组化染色.结果伤后1周接受干细胞移植的治疗组与损伤组相比呈现出明显的运动功能改善,一部分移植到顶叶的干细胞呈Tubulin(+)、GFAP(+),表明它们分化成为神经元或胶质细胞.另外我们观察到治疗组伤区皮层的正常神经元增多,且坏死及凋亡的神经元减少.结论神经干细胞是细胞移植治疗颅脑损伤的一种良好的细胞来源.
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