You Position: Home > Paper
Growth and differentiation of human embryonic neural stem cells boosted by rat Schwann cells
( views:198, downloads:16 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NEUROSURGERY
- Issue:
- 5
- DOI:
- 10.3760/j.issn:1001-2346.2002.05.003
- Key Word:
- 神经干细胞;雪旺氏细胞;分化;免疫组化
Abstract： 目的探讨大鼠雪旺氏细胞对人胚胎神经干细胞生长和分化的作用.方法实验分为三组:组一:干细胞生长于培养雪旺氏细胞1天后的培养液中;组二:干细胞与雪旺氏细胞共培养;组三:干细胞生长于DMEM/F12培养液中.观察干细胞的形态学变化和免疫荧光的表达.结果组一的神经球分化生长,绝大多数细胞tubulin-β阳性,少数为GalC或GFAP阳性;组二中,在雪旺氏细胞生长密集和稀少的地方,干细胞分别表现为不分化和明显分化两种状态;组三的神经干细胞无法正常生长.结论大鼠雪旺氏细胞分泌物支持人胚胎神经干细胞的生长并诱导其分化.
- [1]安沂华,王红云,张相彤,等.大鼠胚胎神经干细胞移植治疗脑出血的实验研究[J].2002,(1).
- [2]万虹,孙梅珍,张亚卓,等.雪旺氏细胞培养方法的比较[J].2001,(9).
- [3]万虹,安沂华,王红云,等.雪旺氏细胞促进共培养大鼠胚胎神经干细胞的分化[J].2002,(2).
- [4]H I, Kornblum,H K, Raymon,R S, Morrison,etc.Epidermal growth factor and basic fibroblast growth factor: effects on an overlapping population of neocortical neurons in vitro.[J].1990,535(2).
- [5]T.J. Kilpatrick,P.F. Bartlett.Cloning and growth of multipotential neural precursors: Requirements for proliferation and differentiation[J].1993,10(2).
- [6]M L, Reynolds,C J, Woolf.Reciprocal Schwann cell-axon interactions.[J].1993,3(5).