Protective effect of icariin on renal ischemia-reperfusion injury in rats and action mechanism

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Author:
FENG Gui-wen(Department of Transplantation, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China)
SHANG Wen-jun(Department of Transplantation, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China)
WANG Yue(Department of Transplantation, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China)
PANG Xin-lu(Department of Transplantation, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China)
LI Jin-feng(Department of Transplantation, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China)
LIU Lei(Department of Transplantation, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China)
Journal Title:
Chinese Journal of Organ Transplantation
Issue:
Volume 33, Issue 09, 2012
DOI:
10.3760/cma.j.issn.0254-1785.2012.09.013
Key Word:
Ischemia reperfusion injury;Oxidoreductases;Icariin;Kidney;Rats

Abstract: Objective To investigate the effect of icariin on renal ischemia-reperfusion injury (IRI) and the action mechanism.Methods SD rats were divided into 3 groups.The model of unilateral renal IRI was established in SD rats,and Icariin (100 mg/kg) was orally administrated by gavage daily from 2 days before operation to 12 days after operation (icariin group).The vehicle of icariin was administrated to IRI model rats as control group,while in the sham-operation group the renal pedicel was only dissociated without treatment.Body weight and kidney function were monitored within 14 days after reperfusion.The kidney was harvested at 24 h after reperfusion,and then malonaldehyde (MDA) and activity of reactive oxygen species (ROS) scavenger enzymes were examined.Histopathological changes were observed at postoperative day (POD) 3 and 14.Results At POD 3,7,11 and 14,the creatinine clearance rate was significantly higher in icariin group than in control group (P<0.01). Icariin group had significantly lower Paller scores which indicated tubules injury than in control group at day 3 after reperfusion (P<0.01 ).In icariin group,MDA level was obviously decreased at 24 h after operation.Compared to control group,icariin group had statistically higher activity of glutathion reductase (GR),catalase and superoxide dismutase (SOD),as well as higher level of reduced glutathione (GSH) (P<0.05).Conclusion Icariin has protective effects on renal IRI,and can promote recovery of kidney function. Icariin can reduce oxidative stress through increasing activity of ROS scavengers.

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