Abstract： Objective To summarize clinical experience in 22 cases of ex vivo split-liver transplantation (SLT). Methods A retrospective analysis was conducted on 22 cases of SL T performed during June 2006 to January 2009. Ten cases were performed at the first phrase (2006. 6-2007. 2), and 12 cases were performed at the second phrase (2009. 1-2010. 1 ). Eleven cadaveric donor livers received ex vivo splitting, and 22 grafts were yielded: 8 cases of extended right lobe, 8 cases of left lateral lobe, 3 cases of right lobe (including middle hepatic vein), and 3 cases of left lobe. The survival rate of patients, recovery of liver function, incidence of vascular complications and biliary complications were observed. Results The median follow-up time of all the patients was 11.7 months (0. 5-43 months). The 1-and 3-survival rate was 82% and 63. 7 % respectively. The incidence of arterial complications was 9. 1%, and biliary complication rate was 33.3 %. Five recipients died, of which 2 cases were related to surgery technology complications. Conclusion With technical improvements and refinements in partial liver transplantation and SLT, SLT can expand the current cadaveric donor pool.