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Study on migration and differentiation of rat bone marrow stem cells to hepatocytes under three different routes of transplantation

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Author:
No author available
Journal Title:
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
Issue:
7
DOI:
10.3760/cma.j.issn.0254-1785.2008.07.006
Key Word:
干细胞移植;肝细胞;大鼠;Stem cell transplantation;Hepatocytes;Rats

Abstract: Objective To observe the migration and differentiation of rat bone rnarmw stem cells to liver under three different routes of transplantation.Methods Intragastric administration was taken to 30 SD rats with CCl4-AAF by which the acute hepatic injured models were made.Bone marrow mononuclear cells which were lsolated from bone marrow with lymphocyte separating medium were labeled by PKH26.The rats were divided into 3 groups.The rats in portal vein-transDlanted group anesthetized were autotransplanted with 0.4 ml bone marrow stem cells suspension via portal vein.The rats in tail vein-transplanted group were autotransplanted with 0.4 ml bone marrow stam cells suspenslon via tail vein.The central sections were made on the abdomen of the rats in hepatic artery-transplanted group to expose the hepatic artery.The distal end of the gastroduodenal arterv was ligated and the blood flow of the common hepatic artery was cut by clamp 0.4 ml bone marrow stem cells suspension was injected In front of ligation of the gastroduodenal artery.Two weeks after transPlantion,the liver tissues were taken from the rats of every group and cut into frozen sections.The immunohistochemistry staining by fluorescein isothiocyanate was done to the sections for detection of the PKH26-labeled cells and the expression of albumin and CK18.Results Red fluorescence of PKH26 in the sections of every group was observed.The number of PKH26-labeled cells in the liver of portal vein-transplanted group,tail vein-transplanted group and hepatic artery-transplanted group was(58.0±2.67),(57.8±3.04)and(58.3±3.52)respectively.There was no significant difference between the groups(P>0.05).The sections in every group expressed albumin and CK18(green fluorescence).The cells expressing red and green fluorescence at the same time were observed under fluorescence microscope(the yellow fluorescence=red fluorescence+the green fluorescence).Conclusion Under injured liver status,bone marrow stem cells of three groups can migrate to the liver and differentiate into hepatocytes.Migration has corresponding effects in three routes of transplantation.

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