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Role of NF-κB pathway in CD40-CD154 interaction-induced human aortic endothelial cell activation

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Author:
No author available
Journal Title:
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
Issue:
4
DOI:
10.3760/cma.j.issn.0254-1785.2008.04.003
Key Word:
NF-κB;内皮细胞;CD40配体;CD154配体;移植;NF-κB;Endothelial cell;CD40 ligand;CD154 ligand;Transplantation

Abstract: Objective To determine human endothelial cells(VEC) activation induced by CD40-CD154 interaction and the role of NF-κB pathway in CD40-CD154 interaction. Methods VEC were isolated from aorta,and subcultures were stimulated by recombinant human TNF-α(rhTNF-α)or IFN-γ(rhIFN-γ). The expression of CD40 and adhesion molecules was detected by using flow cytometry. D1.1 cells expressing CD154 were co-incubated with VEC with or without CD154 specific monoclonal antibody, and the effects of CD40-CD154 interaction in induceding VEC activation were determined. In an additional study,BAY 11-7082,a NF-κB pathway inhibitor,was used to study whether CD40-CD154 interaction-induced VEC activation was NF-κB pathway-dependent. Results Resting VEC demonstrated weak expression of CD40,CD54 and CD106 but undetectable CD62E. VEC stimulated with rhTNF-α or rhINF-γ up-regulated the expression of CD40,CD62E,CD54 and CD106 respectively. VEC demonstrated up-regulation of CD62E, CD54 and CD106 after co-incubation with D1.1 cells,and VEC activation induced bv CD40-CD154 interaction was inhibited by CD154 specific monoclonal antibody. NF-κB could block the VEC activation induced bv rhTNF-α stimulation or by CD40-CD154 interaction. Conclusions The interaction between VEC-derived CD40 and CD154 expressing T cells plays a critical role in VEC activation. Inhibition of NF-κB during CD40-CD154 interaction prevents VEC activation.

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