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Effect of SMO preservation solution on apoptosis and energy metabolism in canine kidneys during cold storage
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 10
- DOI:
- 10.3760/cma.j.issn.0254-1785.2007.10.005
- Key Word:
- 保存液;细胞形态学;凋亡
Abstract： 目的 研究SMO保存液对犬肾低温保存期间细胞凋亡和能量代谢的影响.方法 建立犬离体肾脏单纯低温保存模型,根据保存液的不同分为3组,分别用0～4 ℃HTK保存液(HTK组)、UW保存液(UW组)和自制的SMO保存液(SMO组)对供肾进行灌注和保存;于犬肾保存24、48、72 h后取各组的肾皮质标本,进行组织病理学、细胞凋亡和组织能量代谢的检测.结果 犬肾保存48和72 h时的组织病理学损害,SMO组比HTK组轻,但在各时间点与UW组基本相似.犬肾保存24 h时的细胞凋亡指数,SMO组与HTK组比较,差异无统计学意义(P＞0.05),保存48和72 h后,SMO组显著低于HTK组(P＜0.05);但SMO组与UW组在各时点比较,差异均无统计学意义(P＞0.05).犬肾保存24和48 h时组织中Na+-K+ATP酶的活性,SMO组与HTK组比较,差异无统计学意义(P＞0.05),保存72 h时,SMO组明显高于HTK组(P＜0.05);而SMO组与UW组在保存的各时点差异均无统计学意义(P＞0.05).结论 SMO保存液在减轻细胞形态学改变、减缓肾脏细胞凋亡和保持Na+-K+ATP酶活性方面显著优于HTK保存液,与UW保存液相当.
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