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Inhibitory effects of stachyose on hyperacute rejection in pig-to-human heart xenotransplantation
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 10
- DOI:
- 10.3760/cma.j.issn.0254-1785.2007.10.002
- Key Word:
- 移植物排斥;水苏糖;移植,异种;猪
Abstract： 目的 观察水苏糖对猪到人异种心脏移植超急性排斥反应的抑制作用.方法 以人血液体外灌注猪游离心脏为基础,模拟猪到人异种心脏移植的超急性排斥反应模型.实验分为A、B两组:即分别用人血液灌注及人血液加水苏糖灌注猪离体心脏.观察灌注后两组心脏的跳动时间;体外灌注1 h后,对两组灌注心脏进行免疫组织化学(测定IgG及IgM的沉积)及病理学分析.结果 A组灌注心脏平均跳动时间为(9.5±2.5)min;B组灌注心脏平均跳动时间为(46.8±8.1) min,其中有1个心脏在灌注的1 h内一直跳动;两组心脏跳动时间比较,差异有统计学意义(P＜0.01).A组心肌间质呈弥漫性出血、水肿,血管扩张,内皮细胞肿胀、坏死;免疫组织化学检查显示心肌血管内皮组织中有IgG及IgM沉积.B组心肌间质未见出、凝血和坏死,血管内皮细胞未见肿胀;免疫组织化学检测未见IgG及IgM沉积.结论 水苏糖对异种心脏移植超急性排斥反应具有抑制作用.
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