Prolongation of murine cardiac allograft survival by dendritic cells treated with NF-κB decoy
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 9
- DOI:
- 10.3760/cma.j.issn.0254-1785.2007.09.003
- Key Word:
- NF-κB;诱骗剂;树突细胞;移植物存活;心脏移植
Abstract： 目的 探讨核因子κB(NF-κB)诱骗剂处理供者树突状细胞(DC)对同种异体小鼠移植心脏存活时间的影响.方法 在体外以NF-κB诱骗剂处理供者骨髓来源的DC,并于心脏移植术前7d经门静脉输注2×106个DC给受者,术后1～7 d受者接受亚治疗量的环孢素A(10mg·kg-1·d-1)腹腔注射(联合方案组),并设不作任何处理(对照组)、单用环孢素A(CsA组)、单纯输注未经处理DC(DC对照组)和单纯输注经处理DC(DC实验组)的对照组,另设接受来自第三方供者的对照组(第三供者组,受者的处理同联合方案组),所有受者均接受腹腔心脏移植.观察各组移植心脏的存活时间;采用酶联免疫吸附法检测术后第7天受者血清白细胞介素2(IL-2)、IL-4、IL-10和γ干扰素(IFN-γ)的含量.结果 移植心脏平均存活时间(MST),对照组为7 d,CsA组为10.3 d,DC对照组为7.6 d,DC实验组为21.4 d,联合方案组为53.6 d,第三供者组为9 d,DC实验组移植心脏MST明显长于对照组和DC对照组,差异有统计学意义(P＜0.01);联合方案组移植心脏MST明显长于CsA组、DC实验组及第三供者组,差异有统计学意义(P＜0.01).联合方案组IL-2和IFN-γ的含量最低,而IL-4和IL-10的含量最高,与其它各组比较,差异均有统计学意义(P＜0.05);与对照组、DC对照组及CsA组相比,DC实验组IL-2及IFN-γ的含量也显著降低(P＜0.05),而IL-4和IL-10则升高(P＜0.05).结论 术前输注经NF-κB诱骗剂处理的供者DC可延长同种小鼠移植心脏的存活时间,若术后加用短程亚治疗量CsA,则移植心脏的存活时间得到进一步延长.
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