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Protective effects of low-molecular dextran sulfate on the isolated hearts in mice in ex vivo human serum perfusion model
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 9
- DOI:
- 10.3760/cma.j.issn.0254-1785.2007.09.002
- Key Word:
- 硫酸葡聚糖;血清;血液灌注;小鼠;人类;心室功能,左
Abstract： 目的 研究相对分子质量为5000的硫酸葡聚糖(DXS)对人血清灌注下小鼠离体心脏的保护作用及其可能机制.方法 切取Balb/c小鼠的心脏,采用改良Langendorff灌注装置,以含人血清的灌注液进行心脏灌注,实验组1和实验组2的灌注液中加入DXS(终浓度分别为0.1 mg/ml和2.0 mg/ml),以不含DXS的人血清灌注液为对照组,记录心脏搏动时间,对照组心脏停搏时,同时切取3个组的心脏组织,HE染色,观察组织学变化,免疫组化方法观察心脏组织中补体C3c、C5b-9和IgM、IgG的沉积情况.结果 实验组1心脏搏动时间为(49±15)min,实验组2为(123±19)min,对照组为(17±11)min,两个实验组的心脏搏动时间均显著长于对照组(P＜0.05).HE染色见对照组心肌组织水肿明显,组织间隙变宽,而在灌注相同时间的实验组心脏中此改变不明显.免疫组化染色显示实验组2的心脏组织内C3c和C5b-9沉积较对照组明显减少.结论 低分子质量的DXS对人血清灌注下的小鼠离体心脏具有保护作用,这可能与DXS抑制补体激活,从而抑制补体介导的异种超急性排斥反应有关.
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