Synergistic effect of CsA and DBMC on heterotopic rat cardiac allograft survival
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 7
- DOI:
- 10.3760/cma.j.issn.0254-1785.2007.07.005
- Key Word:
- 环孢菌素;骨髓移植;心脏移植;移植物存活;大鼠
Abstract： 目的 探讨环孢素A(CsA)联合供者骨髓细胞(DBMC)输注对同种大鼠移植心脏存活时间的影响.方法 制作Lewis大鼠到BN大鼠的异位(腹部)心脏移植模型,并按对受者处理的不同分为四组,对照组不进行特别处理,CsA组术后连续7 d给予CsA 5 mg·kg-1·d-1,联合处理组分别于术中及术后第6天输注1×108个DBMC,术后连续7 d给予CsA 5 mg·kg-1·d-1,DBMC组分别于术中及术后第6天输注1×108个DBMC;另设BN大鼠间的心脏移植作为对照(BN对照组).观察移植心脏的存活时间,观测术后第6天血清白细胞介素2(IL-2)、移植心脏组织中肿瘤坏死因子(TNF-α)mRNA表达以及组织学改变,流式细胞仪检测术后第6、12、18天时受者外周血有核细胞中的供者来源细胞、CD3+CD25+细胞、CD4+CD25+细胞的百分比以及共刺激分子CD86表达、CD4+CD45RC+/CD4+CD45RC-等.结果 联合处理组移植心脏存活时间为(21.6±3.2)d,明显长于对照组和DBMC组(P＜0.05),其血清IL-2为(313±95)pg/ml,心肌组织中TNF-α mRNA的表达量为0.12±0.10,均明显低于对照组和DBMC组(P＜0.05),排斥反应程度轻于其它3个移植组.联合处理组大鼠外周血有核细胞上CD86表达受到明显抑制;术后6、12 d,联合处理组的CD4+CD45RC+/CD4+CD45RC-比值低于对照组和DBMC组,CD3+CD25+细胞百分比也低于对照组和DBMC组;接受DBMC输注者外周血中供者来源的有核细胞明显多于未接受DBMC者.结论 短疗程CsA联合DBMC输注能减轻大鼠心脏移植急性排斥反应程度,延长移植心脏存活时间.
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