Protective effects of curcumin or dexamethasone on ischemia-reperfusion injury of rat lung grafts
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 7
- DOI:
- 10.3760/cma.j.issn.0254-1785.2007.07.003
- Key Word:
- 再灌注损伤;肺移植;姜黄素;地塞米松;大鼠
Abstract： 目的 研究姜黄素(CUR)和地塞米松(DXM)对大鼠移植肺缺血再灌注损伤的干预作用.方法 实验分四组进行,CUR组肺移植前3 h供、受者腹腔注射CUR溶液;DXM组肺移植前30min受者腹腔注射DXM溶液;载体组肺移植前3 h供、受者腹腔注射CUR的溶剂二甲基亚砜;假手术组不进行肺移植.每组分别于恢复血液再灌注2 h和24 h各处死大鼠6只,采取颈动脉(CA)血和左肺静脉(LPV,移植侧)血,测定血氧合指数(PO2/FiO2)以及血清中丙二醛(MDA)、总抗氧化能力(TAOC)、肿瘤坏死因子(TNF-α)、白细胞介素6(IL-6)的含量;同时行肺组织病理学观察,测定肺湿重与干重比(W/D),以及肺组织中髓过氧化物酶活性(MPO)、MDA、TAOC、TNF-α、IL-6的含量.结果 再灌注2 h及24 h,CUR组及DXM组LPV血的PO2/FiO2明显高于载体组(P＜0.01).CUR组与DXM组再灌注2 h和24 h的肺水肿病理评分和总分明显低于载体组(P＜0.017),CUR组与DXM组再灌注24 h的W/D明显低于载体组(P＜0.01).再灌注2 h和24 h,CUR组与DXM组肺组织中MPO含量明显低于载体组(P＜0.05,P＜0.01).再灌注2 h时,CUR组和DXM组血清和组织中MDA含量明显低于载体组(P＜0.05),再灌注24 h时,CUR组血清MDA含量和DXM组组织中MDA含量明显低于载体组(P＜0.05).再灌注2 h和24 h,CUR组肺组织和血清中TAOC含量明显高于载体组(P＜0.01,P＜0.05),而DXM组仅再灌注2 h的肺组织和再灌注24 h的血清中TAOC含量高于载体组(P＜0.01).再灌注2 h和24 h,CUR组和DXM组肺组织中TNF-α和IL-6含量低于载体组.结论 CUR和DXM对大鼠移植肺缺血再灌注损伤具有保护作用,其机制可能与二者具有抗氧化和抗炎作用有关.
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