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Induction of T cells expressing Foxp3 by immature dendritic cells in vitro
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.0254-1785.2007.06.005
- Key Word:
- 树突细胞;T淋巴细胞,辅助诱导
Abstract： 目的 探讨不成熟树突状细胞(imDCs)能否在体外诱导T细胞高表达转录因子Foxp3.方法 体外无菌培养BALB/c小鼠骨髓来源的不成熟树突状细胞,并通过脂多糖(LPS)刺激诱导其分化为成熟树突状细胞(mDCs).在96孔细胞培养板中分别以不成熟树突状细胞(imDCs)2×104/孔和成熟树突状细胞(mDCs)2×104/孔与C57BL/6小鼠的脾淋巴细胞以1∶5、1∶10、1∶50、1∶100的比例共同培养6 d.流式细胞术检测混合培养6 d后的淋巴细胞CD4、CD25及转录因子Foxp3的表达.结果 imDCs及mDCs和淋巴细胞混合培养6 d后,淋巴细胞表面CD25的表达水平较正常淋巴细胞明显升高(P＜0.05);mDCs与淋巴细胞混和培养6 d后,淋巴细胞表达Foxp3的水平与正常淋巴细胞差异无统计学意义(P＞0.05),而用imDCs诱导则可使淋巴细胞表达Foxp3显著升高(P＜0.05).结论 imDCs可以在体外诱导T细胞上调转录因子Foxp3的表达.
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