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Effects of hepatic ischemia-reperfusion on intestinal barrier and bacteria translocation in rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.0254-1785.2007.03.005
- Key Word:
- 肝;再灌注损伤;肠黏膜;细菌移位
Abstract： 目的 观察大鼠肝缺血-再灌注后肠黏膜屏障功能的变化,并探讨其对肠源性细菌移位的影响.方法 64只成年健康雄性SD大鼠,随机分为对照组和实验组,每组32只.实验组用无创微血管钳于肝门部夹闭肝动脉、门静脉和胆总管,45 min后去除血管钳,分别在全肝血流阻断45 min后再灌注即刻(0 h)、再灌注1 h、再灌注2.5 h、再灌注4 h共4个时间点采集标本,测定血浆肿瘤坏死因子α(TNF-α)、D-乳酸水平以及肠黏膜中丙二醛、特异性分泌型免疫球蛋白(sIgA)的含量;观察回肠壁组织病理学改变,取肠系膜淋巴结(MLN)、肝、脾、肺、肾及回肠组织匀浆进行细菌培养和鉴定.对照组仅分离门静脉、肝动脉及胆总管,不行血管阻断.结果 实验组在再灌注即刻及再灌注后血浆TNF-α的水平不断升高,再灌注后的TNF-α的水平明显高于再灌注即刻(P＜0.05);D-乳酸的水平也明显高于对照组,但组内不同时间点比较,差异无统计学意义;肠黏膜中丙二醛的含量明显高于对照组(P＜0.05),再灌注后的含量明显高于再灌注即刻(P＜0.05);而sIgA在再灌注即刻及再灌注后明显低于对照组(P＜0.05).随着肝缺血再灌注时间的延长,实验组肠黏膜的病理改变逐渐加重.实验组在再灌注即刻在MLN、肝、脾、肺及肾组织中可培养出细菌,随着再灌注后时间的延长,实验组中MLN、肝、脾、肺及肾组织中检出细菌的动物数明显增多(P＜0.05),培养出的细菌与回肠的优势菌分布一致.结论 肝缺血-再灌注损伤导致肠屏障功能损害,且引起肠道菌群易位.
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