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Effects of IL-12p35 silenced dendritic cells on intestinal allografts survival
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.0254-1785.2007.03.002
- Key Word:
- 器官移植;小肠;树突细胞;转染;大鼠
Abstract： 目的 探讨转染IL-12p35siRNA的供者树突状细胞(DC)对大鼠移植小肠存活时间的影响.方法 在体外以IL-12p35siRNA转染Wistar大鼠骨髓来源的DC,用逆转录聚合酶链反应测定转染细胞IL-12p35mRNA的表达,进行混合淋巴细胞反应,观察DC对SD大鼠T淋巴细胞的刺激作用.制作Wistar大鼠到SD大鼠的节段小肠异位移植模型,转染组小肠移植前7 d经阴茎背静脉给受者注射转染IL-12p35siRNA的DC 4×106个/只;未转染组移植前7 d注射未转染的DC 4×106个/只;对照组注射生理盐水.术后观察受者的存活时间,移植小肠进行组织病理学检查,应用酶联免疫吸附试验检测受者血清中白细胞介素2(IL-2)和γ干扰素(IFN-γ)的水平.结果 DC转染IL-12p35siRNA后,IL-12p35mRNA的表达明显降低,其刺激T淋巴细胞增殖的能力降低(P＜0.01).转染组受者小肠移植后的存活时间为(16.63±3.38)d,明显长于对照组和未转染组(P＜0.01);转染组移植小肠组织中仅有炎症细胞浸润、黏膜层肿胀,绒毛腺体结构和肌层破坏程度较对照组和未转染组明显减轻;术后第10天,转染组的血清IL-2浓度为(175.8±11.5)ng/L,IFN-γ浓度为(70.6±7.2)ng/L,均显著低于对照组和未转染组(P＜0.05).结论 转染IL-12p35siRNA,在体外可减轻DC对T淋巴细胞的刺激作用,在体内可抑制大鼠小肠移植后的排斥反应,延长移植小肠的存活时间.
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