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The biological features of intrahepatic CD4+ CD25+ T cells in the natural tolerance of rat liver transplantation
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.0254-1785.2007.02.001
- Key Word:
- 肝移植;免疫耐受;T淋巴细胞,辅助诱导
Abstract： 目的 研究大鼠肝移植后自发免疫耐受的形成与移植肝内CD4+CD25+调节性T细胞(Tr细胞)的关系.方法 按供、受者不同将实验分为3组.急性排斥组:DA大鼠为供者,LEW大鼠为受者;自发耐受组:LEW大鼠为供者,DA大鼠为受者;同基因组:供、受者均为DA大鼠.各组均建立大鼠原位肝移植模型.分别在肝移植术后4、7、14、30和90 d时采用密度梯度离心法分离移植肝内淋巴细胞,免疫磁珠分离(MACS)法分选出CD4+CD25+Tr细胞;用流式细胞术(FCM)检测细胞纯度,同时分析CD4+CD25+Tr细胞比例的变化;体外细胞增殖试验研究CD4+CD25+Tr细胞对CD4+CD25 T细胞的免疫抑制作用.结果 肝移植早期,急性排斥组和自发耐受组移植肝内CD4+CD25+Tr细胞比例均明显增加,其中急性排斥组增加更为明显;移植后4 d左右,两组CD4+CD25+Tr细胞比例开始下降,急性排斥组的下降幅度较大;移植后30 d,自发耐受组受者的移植肝内CD4+CD25+Tr细胞比例达到第2次高峰,约在移植后90 d时下降至正常生理水平.移植后7 d左右,急性排斥组受者均因发生排斥反应而死亡,而自发耐受组受者均存活.此外,CD4+CD25+Tr细胞能有效抑制CD4+CD25T细胞的增殖.结论 CD4+CD25+Tr细胞是一种具有特异免疫调节功能的T细胞亚群,其主动的免疫抑制功能可能是诱导大鼠肝移植自发免疫耐受的机制之一.
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