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Gene expression profile in acute rejected transplant kidneys detected by microarray
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 11
- DOI:
- 10.3760/cma.j.issn.0254-1785.2006.11.005
- Key Word:
- 肾移植;移植物排斥;基因表达;芯片分析技术
Abstract： 目的 全面认识肾移植急性排斥反应早期移植物内基因表达改变,为进一步探讨肾移植急性排斥反应的发病机理、寻找早期诊断指标提供实验依据.方法 以F344大鼠为供者,近交系Lewis大鼠为受者,建立大鼠原位肾移植急性排斥反应模型(同种异体移植组),以近交系Lewis大鼠间肾移植为对照(近交系移植组).观察术后3、7和14d时的移植肾组织病理学改变,并利用基因芯片技术检测7 d时移植物中基因表达水平,获得其表达谱.结果 急性排斥反应早期存在大量基因变化,在两个通道均有有效杂交信号的基因中,上调的有48条,其中γ干扰素诱导GTP酶基因上调最为显著.结论 移植物排斥反应是个多途径连续衍变的动态不平衡过程,诸多促进排斥与保护因子并存,很难以单一标志物对肾移植急性排斥反应进行早期诊断,但同时通过多个标志物来综合分析还是有一定可能性的.
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