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Effect of Astilbin on expression of CTLA-4 in activated T cells of mouse heart transplantation model with acute rejection
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 10
- DOI:
- 10.3760/cma.j.issn.0254-1785.2006.10.001
- Key Word:
- 心脏移植;落新妇甙;移植物排斥
Abstract： 目的 探讨落新妇甙对混合细胞培养中活化的T细胞产生细胞毒T淋巴细胞相关抗原4(CTLA 4)表达的影响.方法 分离BALB/c小鼠心肌细胞(2×105/ml)和C57BL,/6小鼠脾细胞(1×106/ml),前者作为刺激细胞,后者作为反应细胞,进行混合细胞培养.实验分为3组:(1)对照组,混合细胞培养采用RPMI-1640培养液;(2)落新妇甙组,在RPMI-1640培养液中加入落新妇甙15μg/ml;(3)落新妇甙+CTLA-4单克隆抗体组,在RPMI-1640培养液中加入落新妇甙15μg/ml和CTLA-4单克隆抗体9H10 30μg/ml.原位末端标记(TUNEL)法检测混合细胞培养中T淋巴细胞凋亡情况;逆转录聚合酶链(RT-PCR)和免疫印记(Western blot)法检测T细胞CTLA 4表达情况.结果 落新妇甙组的T细胞凋亡指数明显高于对照组(73.4%±12.5%vs 35.1%±9.2%,P＜0.01),T细胞CTLA-4的表达亦显著高于对照组(P＜0.01).落新妇甙加CTLA-4单克隆抗体组的T细胞凋亡指数和CTLA-4表达与对照组的差异无统计学意义(P＞0.05).结论 落新妇甙诱导心肌细胞排斥反应中活化的T细胞凋亡可能与其增强T细胞中CTLA-4的表达有关.
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