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Donor heart gene transfer of CD40Ig mediated by adenovirus to prolong the survival time of murine cardiac allograft
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 9
- DOI:
- 10.3760/cma.j.issn.0254-1785.2006.09.003
- Key Word:
- 心脏移植;CD40配体;腺病毒科;转染
Abstract： 目的 探讨体外转染CD40Ig融合基因对小鼠移植心脏存活时间的影响.方法 构建携带小鼠CD40胞外段和人IgG Fc融合基因的重组腺病毒载体(AdCD40Ig),以BALB/c小鼠为供者,C57BL/6小鼠为受者,建立小鼠腹部异位心脏移植模型,实验组供心移植前在体外以AdCD40Ig灌注,转染CD40Ig基因,另设空载体转染对照组、非转染对照组和近交系对照组(供、受者均为近交系C57BL/6小鼠).术后观察移植心的存活及移植物中炎症细胞浸润情况,采用酶联免疫吸附试验(ELISA)检测受者体内CD40Ig融合蛋白表达情况,流式细胞仪检测受者体内产生γ干扰素(IFN-γ)的脾细胞.结果 实验组移植心的存活时间达(15.8±0.7)d,明显长于空载体转染对照组和非转染对照组(P＜0.01).术后第2 d,实验组受者体内CD40Ig融合蛋白表达最高,1周后明显降低.术后第7 d,实验组移植心组织中浸润的炎症细胞明显比未处理对照组和空载体对照组少.实验组产生IFN-γ的CD4+和CD8+T淋巴细胞分别为(2.18±0.16)%和(10.82±0.74)%,与近交系对照组接近,明显低于未处理对照组和空载体对照组(P＜0.01).结论 供心体外转染CD40Ig融合基因可有效抑制移植后受者体内同种T淋巴细胞的增殖,并延长移植心的存活时间.
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