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Effects of hVEGF165 gene transfection to pancreatic islets on the revascularization and function of engrafted inbred rats' islets posttransplantation
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 7
- DOI:
- 10.3760/cma.j.issn.0254-1785.2006.07.004
- Key Word:
- 胰岛移植;血管内皮生长因子类;转染;大鼠
Abstract： 目的探讨转染人血管内皮生长因子(VEGF)基因对大鼠胰岛移植物再血管化和生物学功能的影响.方法实验分二步进行,首先构建携带人血管内皮生长因子165(hVEGF165)基因的重组腺病毒载体AdhVEGF165,用其按病毒/细胞的感染倍数(MOI)分别为10、100、500的比例在体外转染新鲜分离、纯化的近交系Lewis大鼠胰岛,检测体外培养的胰岛表达hVEGF165基因的情况;然后按MOI为10的比例在体外用AdhVEGF165转染Lweis大鼠胰岛,再经门静脉注射至近交系Lewis大鼠的肝脏内(VEGF组),并设不含hVEGF165基因的空载体转染对照组(GFP组)和磷酸盐缓冲液处理对照组(PBS液组),术后测定受鼠的血糖变化,进行静脉糖耐量试验(IVGTT),并观察受鼠肝脏中移植胰岛的组织学变化.结果体外转染hVEGF165基因的大鼠胰岛分泌至细胞外的hVEGF165的浓度显著高于未转染者(P＜0.01).糖尿病大鼠移植转染hVEGF165基因的胰岛后,静脉注射葡萄糖后40、60、120 min时的血糖水平显著低于GFP组和PBS液组(P＜0.05);40 min时体循环中胰岛素的浓度,VEGF组显著高于GFP组和PBS液组(P＜0.05);VEGF组移植胰岛内CD34和胰岛素免疫组化染色的强度均高于GFP组和PBS液组.结论转染hVEGF165基因对胰岛移植物的再血管化和生物学功能有促进作用.
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