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The relationship of indoleamine 2, 3-dioxygenase gene expression and acute immune rejection after rat liver transplantation
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 7
- DOI:
- 10.3760/cma.j.issn.0254-1785.2006.07.002
- Key Word:
- 肝移植;移植物排斥;免疫耐受;色氨酸
Abstract： 目的检测吲哚胺2,3双加氧酶(IDO)基因在大鼠肝移植后移植肝中的表达情况,探讨其与急性排斥反应的关系.方法先行大鼠原位肝移植,一组供、受者均为近交系SD大鼠(近交系移植组),另一组为同种移植组,SD大鼠接受Wistar大鼠供肝移植.分别于术后第2、5、7、14 d处死受者,取出移植肝,采用逆转录聚合酶链反应(RT-PCR)、Western印迹法及免疫组化法,检测移植肝组织中IDOmRNA表达、IDO蛋白的水平以及IDO在移植肝细胞中的表达.结果近交系移植组IDO mRNA的表达高峰出现在术后第5 d,至第14 d基本无表达;同种移植组IDOmRNA的表达明显增强,高峰出现在第7 d,持续2周以上.两个组移植肝组织中IDO蛋白的表达与各自的IDOmRNA变化相一致.术后第5、7、14 d,同种移植组IDO阳性细胞的比例明显高于近交系移植组(P＜0.01),强阳性染色主要见于汇管区单个核细胞,以胞浆内染色为主.结论急性排斥反应时IDO基因表达明显增强,IDO的表达水平与急性排斥反应的严重程度一致.
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