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Intra-bone marrow bone marrow transplantation: a novel strategy for the induction of immune tolerance
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.0254-1785.2006.06.004
- Key Word:
- 骨髓移植;免疫耐受;皮肤移植
Abstract： 目的探讨异基因小鼠髓腔内骨髓移植(IBM-BMT)诱导免疫耐受的效果.方法雄性BALB/c小鼠和雌性C57BL/6小鼠分别作为骨髓移植的供、受者.受者预处理后进行IBM-BMT,建立异基因小鼠骨髓移植模型.通过皮肤移植和混合淋巴细胞反应(MLR)对受者的耐受状态进行检测.结果接受过IBM-BMT的受者进行供者来源的皮肤移植,移植物的存活时间＞300 d,较对照组的(12.7±1.63)d明显延长(P＜0.01),而受者接受来自无关供者(KM小鼠)的皮肤移植物存活时间未见延长.接受过IBM-BMT的受者脾细胞对供者脾细胞的MLR增殖率均明显降低,与对照组比较,P＜0.01,而对无关供者的脾细胞仍表现强烈的增殖反应.结论应用IBM-BMT可以诱导受者获得供者抗原的特异性免疫耐受,使移植物存活时间延长.
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