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An experimental study on the role of CTLA4Ig gene transfected DCs in the induction of immune tolerance
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.0254-1785.2006.06.002
- Key Word:
- 转基因;树突细胞;T淋巴细胞,抑制效应
Abstract： 目的探讨转染人细胞毒性T淋巴细胞相关抗原4免疫球蛋白(CTLA4Ig)基因的树突状细胞(DCsRev)对反应性T细胞的作用.方法通过重组逆转录病毒将目的基因CTLA4Ig转染到大鼠骨髓来源的树突状细胞(DCs)中.采用逆转录聚合酶链反应(RT-PCR)和斑点酶联免疫吸附试验(Dot-ELISA)检测CTLA4Ig在DCs中的表达;采用混合淋巴细胞反应(MLR)检测DCsRev对反应性T细胞的作用.结果检测结果证实CTLA4Ig基因成功转染至DCs.DCsRev的数量以及用DCsRev预处理反应性T细胞的时间长短与抑制MLR中T细胞增殖有一定的效应关系.当DCsRev数量在103～104之间时,其抑制率最高,抑制率可达到为69.12%;用DCsRev预处理反应性T细胞12 h时,其对MLR中T细胞增殖的抑制率最高,为98.3%,12 h以后,随着预处理时间的延长,抑制率却不断下降.经DCsRev诱导的大鼠体内脾淋巴细胞在MLR中增殖低下.结论转染CT-LA4Ig基因的DCs不但丧失了刺激MLR的能力,并且能够抑制MLR中反应性T细胞的增殖,提示DCsRev可能诱导抗原特异性T细胞的免疫耐受.
- [1]Miao G,Ito T,Uchikoshi F,etc.Development of donor-specific immunoregulatory T-cells after local CTLA4Ig gene transfer to pancreatic allograft.[J].2004,78(1).
- [2]Ikeda S,Ono J,Matsuoka N,etc.Beneficial effects of costimulatory blockade with anti-inducible costimulator antibody in conjunction with CTLA4Ig on prevention of islet xenograft rejection from rat to mouse.[J].2004,78(11).
- [3]Furukawa H,Hua N,Yamashita K,etc.Gene therapy-mediated CD40L and CD28 co-stimulatory signaling blockade plus transient anti-xenograft antibody suppression induces long-term acceptance of cardiac xenografts.[J].2004,78(10).