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Changes of CD45RC+/CD45RC- ratio in peripheral blood following small bowel transplantation in rats and significance
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 7
- DOI:
- 10.3760/cma.j.issn.0254-1785.2005.07.005
- Key Word:
- 移植;小肠;移植物排斥;抗原,CD45;大鼠
Abstract： 目的探讨可用于诊断小肠移植后排斥反应的指标.方法采用F334大鼠建立同系全小肠移植模型,以近交系F334大鼠和BN大鼠建立同种全小肠移植模型,术后采用流式细胞仪连续测定外周血CD4+、CD8+细胞中CD45+亚群以及CD45RC+细胞与CD45RC-细胞比值(CD45RC+/CD45RC-)的变化,同期进行移植肠组织病理学检查.结果同系移植组受者术后均获长期存活(＞28 d);同种移植组受者术后存活(12.0±1.3)d,出现中、重度排斥反应.术后外周血CD4+CD45+和CD8+CD45+细胞的变化,两组间的差异无统计学意义;同系移植组CD4+CD45RC+/CD4+CD45RC―及CD8+CD45RC+/CD8+CD45RC―在术后3 d升高,然后迅速降至手术当天的水平,同种移植组上述指标术后均维持较高水平.结论动态观察CD4+CD45RC+/CD4+CD45RC-较适于监视排斥反应的发展过程,而CD8+CD45RC+/CD8+CD45RC-更适于排斥反应的早期诊断.
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