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Evaluation of the viability and energy metabolism of canine pancreas graft subjected to significant warm ischemia damage
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 7
- DOI:
- 10.3760/cma.j.issn.0254-1785.2005.07.004
- Key Word:
- 胰腺移植;移植物存活;功能恢复;腺苷三磷酸
Abstract： 目的探讨热缺血时间对胰腺能量代谢状态及胰腺移植后移植物功能的影响.方法切取犬胰腺左叶,按分组要求,胰腺分别经历30、60、90、120 min热缺血,以0～4 ℃ UW液灌洗,并保存24 h,然后行胰腺节段自体异位移植,同时设正常对照组和未经历热缺血的胰腺节段自体移植组.术后连续观察血糖、静脉葡萄糖耐量试验(IVGTT)、血液中胰岛素含量,测定术后24 h和1周时胰液中淀粉酶浓度,高效液相色谱法检测移植前胰腺组织中ATP和腺苷核苷酸总量(TAN),并观察移植后1 h时移植物的组织学变化.结果热缺血30 min组和60 min组的术后3～5 d血糖即可恢复至正常范围,而热缺血90 min组和120 min组术后1周血糖未能恢复至正常;术后1周,无热缺血组、热缺血30 min组和60 min组的IVGTT K值大于1,热缺血90 min组和120 min组小于1,且其静脉血中胰岛素的浓度明显低于无热缺血组、热缺血30 min组和60 min组(P＜0.05);热缺血30 min组和60 min组胰腺组织中ATP和TAN的含量明显高于热缺血90 min组和120 min组(P＜0.05);无热缺血组以及热缺血30 min组和60 min组的胰腺组织结构基本正常,而热缺血90 min组和120 min组的组织破坏明显,腺泡坏死、出血和炎症细胞浸润严重.结论用于移植的胰腺热缺血30～60 min是安全的,移植后胰腺功能迅速恢复正常;超过60 min的热缺血对移植物的能量代谢有显著负面影响.
- [1]G. Florack,D.E.R. Sutherland,R. Ascherl,etc.Definition of normothermic ischemia limits for kidney and pancreas grafts[J].1986,40(6).
- [2]Haim Paran,Joseph Klausner,Annette Siegal,etc.Effect of the Somatostatin Analogue Octreotide on Experimental Pancreatitis in Rats[J].1996,62(2).
- [3]F O, Belzer,J H, Southard.Principles of solid-organ preservation by cold storage.[J].1988,45.
- [4]Humar A,Kandaswamy R,Drangstveit MB,etc.Prolonged preservation increases surgical complications after pancreas transplants.[J].2000,127(5).
- [5]A, Wakai.Effect of adenosine on ischaemia-reperfusion injury associated with rat pancreas transplantation.[J].2002,89.
- [6]TAKASHI ORII,NOBUHIRO OHKOHCHI,SUSUMU SATOMI,etc.ASSESSMENT OF LIVER GRAFT FUNCTION AFTER COLD PRESERVATION USING31P AND23Na MAGNETIC RESONANCE SPECTROSCOPY[J].1992,53(4).