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Protective effect of inhibiting the activation of nuclear factor-κB by dexamethasone on donor rat lung
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 7
- DOI:
- 10.3760/cma.j.issn.0254-1785.2005.07.002
- Key Word:
- 氟米松;肺;NF-κB;再灌注损伤;大鼠
Abstract： 目的探讨核因子-κB(NF-κB)抑制剂氟美松对大鼠供肺缺血-再灌注损伤的保护作用.方法 24只大鼠随机分为两组,实验组采用含氟美松的低钾右旋糖酐(LPD)液灌洗和保存供肺,对照组以LPD液灌洗和保存供肺,16 h后建立离体肺再灌注模型,循环灌注1 h.再灌注期间,每隔15 min测定供肺氧合后动脉血氧分压(PaO2)和气道峰压(PawP);再灌注结束后测定肺组织湿重与干重比(W/D)以及肺组织中髓过氧化物酶(MPO)活性、细胞间粘附分子(ICAM-1)和NF-κB的表达.结果再灌注15 min后,两个组的PaO2逐渐降低、PawP逐渐升高,但实验组PaO2的降低程度和PawP的升高程度明显低于对照组(P＜0.01);再灌注后,实验组的W/D和MPO明显低于对照组(P＜0.01),其肺组织中ICAM-1、ICAM-1 mRNA、NF-κB及NF-κB mRNA的表达量也明显低于对照组(P＜0.01).结论应用氟美松抑制NF-κB的表达对大鼠供肺缺血-再灌注损伤具有保护作用.
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