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Effect of intracoronary adenoviral mediated gene transfer of transforming growth factor β1 on acute vascular rejection of discordant cardiac xenograft
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 7
- DOI:
- 10.3760/cma.j.issn.0254-1785.2005.07.001
- Key Word:
- 心脏移植;移植,异种;转化生长因子β;基因转移;移植物排斥
Abstract： 目的探讨移植物局部转染转化生长因子β1(TGF-β1)基因对非协调性异种心脏移植急性血管性排斥反应(AVR)的影响.方法建立豚鼠到SD大鼠的颈部心脏移植模型,移植前受鼠接受中华眼镜蛇毒因子和环孢素A预处理,供心切取后,经冠状动脉按每克心肌组织灌注5×1010 PFU携带TGF-β1基因的重组腺病毒载体进行基因转染,再移植到经过预处理的SD大鼠颈部(基因转染组),另设灌注5×1010 PFU腺病毒空白载体的空白载体组和对照组.术后观察各组移植物的存活情况、移植物组织学变化情况以及移植物中CD68和CD57的表达、细胞凋亡指数、TGF-β1的表达.结果基因转染组移植物的存活时间为(95±3)h,明显长于空白载体对照组的(57±2)h和对照组的(60±2)h(P＜0.01);各组移植物均呈急性血管性排斥反应的病理改变,但基因转染组较轻;基因转染组移植物组织中炎症细胞浸润数、CD68和CD57的表达量及细胞凋亡指数明显低于空白载体对照组和对照组,并能检测到外源性TGF-β1的表达.结论经冠状动脉灌注重组腺病毒载体介导的TGF-β1基因转移可减轻非协调性异种心脏移植AVR,明显延长移植物的存活时间.
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