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The inhibitory effects of a novel HLA-derived peptide on the immune function of rat splenocytes
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.0254-1785.2005.02.005
- Key Word:
- 组织相容性抗原;血红素氧化酶
Abstract： 目的观察一种新型组织相容性白细胞抗原(HLA)衍生肽(RDP1258)的免疫抑制作用并探讨其机制.方法人工合成一种HLA-RDP1258,用3H胸腺嘧啶(3H-TdR)掺入法观察其在体外对大鼠脾细胞增殖反应的影响;以酶化学法观察其对脾细胞血红素氧合酶-1(HO-1)活性的影响.并与HLA衍生肽(HLA-2702.75-84)进行对比.结果HLA-RDP1258抑制大鼠脾细胞增殖作用比HLA-B2702.75-84强,两者比较,差异有统计学意义(P＜0.05);HLA-RDP1258对淋巴细胞增殖具有显著的抑制作用,抑制率大约是HLA-B2702.75 84的8倍以上;HLA-RDP1258具有较明显的体外HO-1抑制作用,是HLA-B2702.75-84抑制作用的5倍以上,呈剂量依赖性.结论HLARDP1258能够显著抑制大鼠脾细胞经丝裂原或同种抗原刺激引起的增殖反应,这种作用可能与影响HO-1活性有关.
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