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Optimal time points for transplantation with cultured dopaminergic cells
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.0254-1785.2005.02.004
- Key Word:
- 细胞培养;多巴胺;神经元;细胞移植;帕金森病
Abstract： 目的探索体外培养的多巴胺能神经细胞的最佳移植时机,为细胞移植治疗帕金森病奠定基础.方法体外扩增E11胎鼠腹侧中脑神经前体细胞7d后,分别在诱导分化期开始0、1、2、3、4、5、6、7d时收获细胞,重新接种后再培养7 d.根据多巴胺能神经元生存率高低,初步决定出最佳移植时机.结果在诱导分化期开始0d和1d传代细胞后,多巴胺能神经元的生存率显著高于其他时间点.结论E11胎鼠腹侧中脑神经前体细胞体外扩增后诱导分化0d和1 d时,可能是最佳移植时机.
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