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Influence on donor heart preservation and mechanism of adenosine A1 receptor-induced delayed preconditioning
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.0254-1785.2004.04.005
- Key Word:
- 腺苷;移植预处理;器官保存;超氧化物歧化酶
Abstract： 目的探讨腺苷A1受体激动剂预处理延迟效应对保存大鼠心脏的影响及其机制.方法Wistar大鼠随机分为8组,A、C组以高选择性腺苷A1受体激动剂(CCPA)预处理;D、E、F组在CCPA预处理前分别注射锰-超氧化物歧化酶(M n-SOD)反义、有意义、错配寡核苷酸(ODN);B、G组注射生理盐水,H组只注射反义寡核苷酸.24 h后,A、B组用4℃St.Thomas液保存4 h,复灌1 h,而另6组采取低温缺血3 h,复灌1 h.观测心功能、磷酸肌酸激酶(CK-mb)、三磷酸腺苷(ATP)含量等.结果A组左室内压上升与下降最大速率恢复率(±dp/dtmax,%)为62.83±17.27,66.81±18.99,心肌ATP含量(10-3μmol/g)为3.67±1.42;而B组分别为40.41±18.29,44.70±25.14,1.46±0.54;A组均明显高于B组,差异均有显著性(P＜0.01 or P＜0.05).C组±dp/dtmax恢复率、ATP、Mn-SOD活性均明显高于D、G、H组(P＜0.01 or P＜0.05),而与E、F组比较,差异无显著性.结论腺苷A1受体激动剂能诱导预处理的延迟效应,改善离体大鼠心脏的保存效果,而该效应与Mn-SOD的高表达有关.
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