You Position: Home > Paper
Prolonged myocardial protection of 11, 12-epoxyeicosatrienoic on immature isolated rabbit hearts
( views:222, downloads:9 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.0254-1785.2004.04.004
- Key Word:
- 器官保存液;心脏停搏,人工;兔
Abstract： 目的观察11,12-EET(11,12-epoxyeicosatrienoic acid)液对未成熟兔离体心脏的保护作用.方法将48只未成熟离体兔心按随机分配原则分成对照组(24只)和EET组(24只),对照组保存心脏采用St.Thomas No.2液为停搏液及保存液,EET组采用St.Thomas No.2液+11,12-EET为停搏液及保存液,分别保存心脏8 h、16 h、24 h.利用非循环式Langendorff灌注装置测定各组心脏保存前及保存后再灌注30 min(37℃)的左心室变化压(LVDP)、舒张末期压(LVEDP)、最大压力变化速率(+dp/ptmax)和冠状动脉流量(CBE),并测定心律失常评分、心肌肌酸激酶(CK)、乳酸脱氢酶(LDH)含量及观察心肌超微结构的改变等指标.结果(1)EET组各保存时间的心功能恢复率、心肌水肿程度、心律失常评分均好于相应对照组;CK、LDH及心肌超微结构的改变亦明显优于对照组.(2)心脏保存16 h后,EET组的心功能恢复率、心律失常评分基本接近心脏保存之前的测定值,而对照组则明显减低.(3)心脏保存24 h,EET组心脏全部复跳,对照组5只心脏不能复跳.结论St.Thomas No.2液中加入11,12-EET可延长对未成熟兔离体心脏的保存时间并增强其保存效果.
- [1]S, Hu,H S, Kim.Activation of K+ channel in vascular smooth muscles by cytochrome P450 metabolites of arachidonic acid.[J].1993,230(2).
- [2]C F, Vahl,A, Bonz,C, Hagl,etc."Cardioplegia on the contractile apparatus level": evaluation of a new concept for myocardial preservation in perfused pig hearts.[J].1995,43.
- [3]K G, Proctor,J R, Falck,J, Capdevila.Intestinal vasodilation by epoxyeicosatrienoic acids: arachidonic acid metabolites produced by a cytochrome P450 monooxygenase.[J].1987,60.
- [4]Mason RP,Graham L,Yang B,etc.Overexpression of cytochrome P450 CYP2J2 protects against hypoxia-reoxygenation injury in cultured bovine aortic endothelial cells.[J].2001,60(2).
- [5]C L, Oltman,N L, Weintraub,M, VanRollins,etc.Epoxyeicosatrienoic acids and dihydroxyeicosatrienoic acids are potent vasodilators in the canine coronary microcirculation.[J].1998,83.
- [6]M J, Walker,M J, Curtis,D J, Hearse,etc.The Lambeth Conventions: guidelines for the study of arrhythmias in ischaemia infarction, and reperfusion.[J].1988,22.
- [7]Zeldin DC.,Chen WN.,Steenbergen C. Falck JR.,etc.Molecular Cloning, Expression, and Functional Significance of a Cytochrome P450 Highly Expressed in Rat Heart Myocytes[J].1997,272(19).
- [8]M J, Curtis,M J, Walker.Quantification of arrhythmias using scoring systems: an examination of seven scores in an in vivo model of regional myocardial ischaemia.[J].1988,22.
- [9]Tanya Ravingerova,Narcis Tribulova,Jan Slezak,etc.Brief, intermediate and prolonged ischemia in the isolated crystalloid perfused rat heart: Relationship between susceptibility to arrhythmias and degree of ultrastructural injury[J].1995,27(9).