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Protective effect of glucocorticoid-free immunosuppressive regimen on allogenic islet transplantation
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.0254-1785.2004.04.002
- Key Word:
- 免疫抑制剂;胰岛移植;糖皮质激素;大鼠
Abstract： 目的评价非糖皮质激素的免疫抑制方案对防止大鼠同种异体胰岛移植排斥反应的效果.方法大鼠同种异体胰岛移植后,分别应用他克莫司(FK506)+霉酚酸酯(MMF)和FK506+MMF+泼尼松(Pred)行免疫抑制治疗两周,并设对照组,动态观察受者血糖、胰岛素及C肽变化,并作移植部位的形态学检测.结果FK506+MMF组和FK506+MMF+Pred组与对照组相比,移植胰岛存活时间明显延长,移植后2个月在受者肝汇管区可见较多形态完整的胰岛细胞.FK506+MMF组维持术后正常血糖、胰岛素及C肽的时间超过60 d,而FK506+MMF+Pred组与前者比较,分泌C肽较少(P＜0.05),血糖维持在较高水平(P＜0.01),胰岛素水平两组差异无显著性.FK506+MMF+Pred组停药两周以后的血糖水平较用药期间、停药两周内有明显降低(P＜0.05),胰岛素和C肽分泌有所增多,但差异无显著性.结论应用FK506+MMF和FK506+MMF+Pred均有很强的免疫抑制效应,但糖皮质激素对胰岛细胞有毒性作用.小剂量FK506与MMF联用对移植胰岛细胞有较强的保护作用.
- [1]胡远峰,张洪,徐静娟,等.大鼠胰腺的胰岛分离与纯化[J].1990,(2).
- [2]Tzakis A,Tsaroucha A,Olson L,etc.Evidence that tacrolimus augments the bioavailability of mycophenolate mofetil through the inhibition of mycophenolic acid glucuronidation.[J].1999,21(1).
- [3]Debonera Fotini,Aldeguer Xavier,Que Shingyi,etc.Glucocorticoids Inhibit Progression of Cell Cycle Pathways Following Ischemia\/Reperfusion(I\/R)injury in the Liver.[J].2000.
- [4]T, Imamura,M, Koffler,J H, Helderman,etc.Severe diabetes induced in subtotally depancreatized dogs by sustained hyperglycemia.[J].1988,37.
- [5]LAKSHMI K. GAUR,Y. NITTA,E. KENNEDY,etc.Induction of Islet Allotolerance in Nonhuman Primates[J].2002,958(1).
- [6]Marsh CL,Paty BW,Robertson RP,etc.Inhibitory effects of immunosuppressive drugs on insulin secretion from HIT-T15 cells and Wistar rat islets.[J].2002,73(3).
- [7]Ogawa A,Johnson J H,Ohneda M,etc.Roles of insulin resistance and beta-cell dysfunction in dexamethasone-induced diabetes.[J].1992.
- [8]Yasunami, Yohichi,Ryu, Shinichiroh,Ueki, Masao,etc.Donor-Specific Unresponsiveness Induced by Intraportal Grafting and Fk506 in Rat Islet Allografts: Importance of Low Temperature Culture and Transplant Site on Induction and Maintenance[J].1994,3(1).