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A comparative study of hair microarray and blood monoclonal antibody in HLA-Ⅱ typing
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.0254-1785.2004.04.001
- Key Word:
- 基因芯片;抗体,单克隆;寡核苷酸芯片
Abstract： 目的同步双盲法研究单克隆抗体法和基因芯片法在HLA分型中的准确性、重复性和实用性.方法研究样本46份,单克隆抗体法采用一步法单抗分型技术,基因芯片法采用寡核苷酸芯片技术.结果46份标本的单克隆抗体法和基因芯片法分型成功,对有差异的结果进行基因芯片法复查,重复率100%.单克隆抗体法耗时1.5 h,基因芯片法耗时3.5 h.基因芯片法的漏检率低于单克隆抗体法.两种方法主要在对DR2(DR15,DR16)的检测结果上不一致.结论目前HLA-Ⅱ类抗原的分型,尤其是临床大样本分型,可以采用单克隆抗体分型技术进行快速分型.对于要求精细配型的骨髓移植,应该采用基因芯片分型或其他DNA分型技术.对于单克隆抗体分型结果可疑的样本应用基因芯片分型确认.
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