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Genescan analysis of expression and clonality of TCR Vβ repertoire T cell in renal transplant patients in early stage
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.0254-1785.2004.01.005
- Key Word:
- 肾移植;T淋巴细胞;基因,T细胞受体;基因表达
Abstract： 目的了解肾移植患者移植前后外周血单核细胞T淋巴细胞受体(TCR)Vβ基因谱系的变化和是否存在T淋巴细胞的特征性克隆增殖.方法通过逆转录聚合酶链反应(TR-PCR)扩增11例首次肾移植患者术前及术后外周血单核细胞TCR Vβ24个亚家族基因,了解其表达情况;阳性的PCR产物进一步经荧光素标记和基因扫描的方法分析TCR的互补决定区3(CDR3)的长度及序列.结果 11例患者术前分别表达1～10个TCR Vβ亚家族,大部分为多克隆;术后第20 d分别表达1～5个亚家族,绝大部分为寡克隆;大部分TCR Vβ亚家族与术前相同.发生急性排斥反应的1例在排斥反应冲击治疗前无TCR Vβ亚家族表达,冲击治疗过程中表达5个Vβ亚家族,排斥逆转后表达2个Vβ亚家族(多克隆);1例肾功能恢复延迟者在恢复中表达4个Vβ亚家族,恢复后表达5个Vβ亚家族,以多克隆为主.结论肾移植患者术前外周血T淋巴细胞存在着TCR Vβ亚家族倾斜分布的现象,术后20 d倾斜分布更明显;急性排斥反应与某些特异性T淋巴细胞克隆有关,随着排斥反应的逆转,这些克隆随之消失;排斥反应逆转和肾功能恢复正常后的多克隆表达可能提示免疫状态趋于正常化.
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