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Immune tolerance induction by IL-10 modified dendritic cells in rat small bowel transplantation
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.0254-1785.2003.06.103
- Key Word:
- 白细胞介素10;小肠;移植;免疫耐受
Abstract： 目的探讨白细胞介素10(IL-10)修饰的供者树突状细胞(DC)对大鼠小肠移植术后免疫耐受的诱导效果,为抗排斥反应治疗提供依据.方法健康成年SD大白鼠为供者,Wistar大鼠为受者.受者大鼠18只,随机分为3组,每组均为6只.A组:为对照组,受者不经任何预处理,即行小肠移植术;B组:小肠移植前7d,每只受者经尾静脉注射供者的DC,细胞数为2×107个; C组:小肠移植前7d,每只受者经尾静脉注射用IL-10修饰的供者DC,细胞数为2×107个.B、C组1周后行大鼠异位节段性小肠移植术,观察各组受者移植小肠存活情况.结果 A、B、C组大鼠移植小肠存活时间分别为:(7.33±2.42)d、(8.33±2.94)d、(18.5±5.17)d.经统计学分析,A、B组之间差异无显著性,而C组与A、B组比较,差异均有显著性(P<0.01).结论用IL-10修饰的供者树突状细胞对受者进行预处理,可明显延长受者大鼠小肠移植术后存活时间.
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